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KMID : 0361020000430060610
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Korean Journal of Otolaryngology - Head and Neck Surgery
2000 Volume.43 No. 6 p.610 ~ p.614
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Expression of Inducible Nitric Oxide Synthase and Its Steroid Effect in TDI-Induced Nasal Hyperreative Guinea Pigs
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이승균/Seung Kyun Lee
강준명/조진희/류찬/원유성/윤희로/Junn Myung Kang/Jin Hee Cho/Chan Rhyoo/Yu Sung Won/He Ro Yoon
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Abstract
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-Abstract-
Background and Objectives : Nitric oxide (NO) in exhaled air is elevated in allergy. Topical corticosteroid
therapy which gas been shown to reduce airway inflammation is associated with reduction in exhaled
levels of NO in allergy. The aim of this study is to investigate the induction of inducible nitric oxide
synthase (iNOS) and the effect of steroid on the expression of iNOS in the nasal mucosa of TDI (toluene
diisocyanate)-induced nasal hyperreactive guinea pig. Materials and Methods : We developed an allergy
model in guinea pigs using the intranasal application of TDI. We evaluated the iNOS expression and in
vivo effects of triamcinolone on the expression of iNOS and infiltration of eosinophil in TDI-sensitized
guinea pigs by immunohistochemical stain. Results : Nasal symptoms were significantly suppressed and
the number of eosinophils in the nasal mucosa were significantly inhibited by the treatment of
triamcinolone. Immunoreactivity to iNOS was localized to ciliated cells of epithelium, vascular endothelial
cells, secretory cells of nasal glands and some inflammatory cells in the mucosa of the control group.
High expression of iNOS in the nasal mucosa of the TDI-sensitized group was demonstrated, and it was
suppressed by triamcinolone therapy. Conclusion : These results show that increased expression of iNOS
may contribute to allergic inflammation and the antiinflammatory effect of steroid in allergy is partly
mediated by the reduction of iNOS expression.
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